June 21, 2012
JoVE is proud to announce a novel technique to count and characterize mammalian cells using the Moxi Z Mini Automated Cell Counter from Orflo Technologies. The video article demonstrates proper use of the device, and has been sponsored by Orflo Technologies, and can be found here.
Orflo Technologies ( www.orflo.com ), founded in 2003, is committed to developing and commercializing highly accessible technologies designed to disrupt and significantly improve the pace of life science research. Orflo launched their breakthrough Moxi Z technology in July of 2011, which is the world's first cell analyzer enabling a highly precise and accurate three parameter analysis in 8 seconds; count, mean cell volume and an indicator of cell health through Orflo's proprietary Moxi Population Index. MoxiZ is already the recipient of a prestigious industry award through and independent third party and is driving efficiencies in many top academic and biopharmaceutical research labs across the globe. Orflo is constantly innovating and has plans to launch future disruptive technologies with similar form, fit and function to the Moxi Z.
The ability to count cells or particles in a sample is valuable for many clinical and research applications, including hematological analysis, routine cell culture and industrial controls. Mechanical and electronic techniques have been used to count cells since Wallace H. Coulter first established his eponymous principle in 1953, when he first patented the means to count particles suspended in a fluid. Since then, the technique has been used and modified, becoming a gold standard in many laboratories. The Moxi Z Mini Automated Cell Counter applies the Coulter Principle by passing a sample of cells through a thin film membrane, which also characterizes the health of the cell and the size of the cell.
"Through the integration of state of the art semi-conductor technology, microfluidics, proprietary software algorithm development and advancements in thin film manufacturing, we at Orflo have been able to revolutionize a critical step in cell culture and maintenance through our Moxi Z cell analysis platform,” tells Ted Ayliffe of Orflo Technologies. He continues, “Ultimately our mission is to greatly improve the efficiency and results of customer’s research with the goal of accelerating their scientific journey’s."
When asked why the Moxi Z is superior to other technologies, author Gregory Dittami responded, “Other technologies have attempted to simply automate the hemocytometer approach to counting and, in doing so, have mirrored the error and variability inherent in that method. In contrast, the Moxi Z has implemented the universally-regarded, "gold-standard" of counting technologies, the Coulter Principle. Particles are hydrodynamically focused through a detection aperture and the volume of each cell is precisely measured to ensure consistently accurate count and sizing information across a broad dynamic range.”
This publication is significant for JoVE as it marks continued publication of procedures using cutting edge technologies with a wide application in the life sciences. More information on JoVE’s partnership with the scientific industry can be found here.
